写一个python代码通过全基因组和注释文件筛选每个基因对应的最长转录本
时间: 2023-06-04 20:04:26 浏览: 135
以下是 Python 代码实现的示例:
```python
# 导入必要的库
import pandas as pd
# 读取全基因组文件和注释文件,例如:
genome_file = 'genome.fasta'
annot_file = 'annotation.gff3'
genome = open(genome_file, 'r').read()
annot = pd.read_csv(annot_file, sep='\t', header=None, comment='#')
# 筛选出所有基因的注释
gene_annot = annot[annot[2] == 'gene']
gene_annot.columns = ['seqid', 'source', 'type', 'start', 'end', 'score', 'strand', 'phase', 'attributes']
# 遍历每个基因,找到对应的最长转录本
for gene in gene_annot.itertuples():
start = gene.start
end = gene.end
transcripts = annot[(annot[2] == 'mRNA') & (annot[0] == gene.seqid) & (annot[3] >= start) & (annot[4] <= end)]
if not transcripts.empty:
longest_transcript = transcripts.loc[transcripts[4].idxmax()]
print(longest_transcript.attributes)
else:
print('No transcripts found for gene', gene.attributes['ID'])
```
此代码将在控制台输出每个基因对应的最长转录本的属性。此示例仅供参考,具体实现可能因数据和注释格式而异。
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