roary：-------------------- WARNING --------------------- MSG: Got a sequence without letters. Could not guess alphabet

roary是一个用于进行基因组学分析的工具，这个警告通常是由于在运行roary时遇到了没有序列信息的数据，导致无法推测序列类型而产生的。您可以检查一下您的输入数据，确认您的FASTA文件是否包含了正确的序列信息。如果您的FASTA文件中确实存在一些空序列或者没有序列信息的记录，那么这些记录可能会被roary忽略，导致分析结果不准确。建议您先对输入数据进行预处理，去除空序列或者没有序列信息的记录，以确保分析结果的准确性。

相关问题

warning: ----------------- SECURITY WARNING ---------------- warning: | TLS certificate verification has been disabled! | warning: --------------------------------------------------- warning: HTTPS connections may not be secure. See https://aka.ms/gcm/tlsverify for more information. warning: ----------------- SECURITY WARNING ---------------- warning: | TLS certificate verification has been disabled! | warning: --------------------------------------------------- warning: HTTPS connections may not be secure. See https://aka.ms/gcm/tlsverify for more information. To https://gitee.com/serendipity-bb/git_study.git ! [rejected] master -> master (non-fast-forward) error: failed to push some refs to 'https://gitee.com/serendipity-bb/git_study.git' hint: Updates were rejected because the tip of your current branch is behind hint: its remote counterpart. Integrate the remote changes (e.g. hint: 'git pull ...') before pushing again. hint: See the 'Note about fast-forwards' in 'git push --help' for details.

这个警告提示说明 TLS 证书验证已被禁用，HTTPS 连接可能不安全。你可以访问 https://aka.ms/gcm/tlsverify 获取更多信息。另外，这个错误提示说明推送被拒绝，因为你当前分支的提交落后于远程分支的提交。在再次推送之前，请先整合远程的修改（例如，运行 'git pull ...'）。你可以查看 'git push --help' 中关于快进合并的说明。