电子声热波显微镜实现生物组织分层定量成像 - CSDN文库

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第

46

卷第

6

期

2010

年

11

月

南京大学学报(自然科学)

JOURNAL

OF NAN

]l

NG

UNIVERSITY

(NATURAL

SCIENCES)

Quantitatively laminated imaging by scanning

electron-acoustic

thermal

wave microscopy

Vo

l.

46

,

No.6

Nov.

,

2010

Zhu

Shou-Jing

l

,

Zhang

Shu-Yi

l

川

，

Gao

Chun-

儿

1ing

l

，

Zhang

Zhong-Ning

l

,

Shui

Xiu-Ji

l

,

Zhang

Xiu-Ling

2

,

She

Wωz-Ðoηg2

(1.

Laboratory

of

Modern

Acoustics.

Institute

of

Acous

tI

cs.

Nan

]i

ng

University.

Nanjmg.

210093

,

Chma;

2.

Nanjing

Gulou

Hospltal

,

Medlcal

School

of

NanJing

Universlty.

Nanjing.

210008

,

China)

Abstract:

Lammated

images

of

microstructures

of

b

lO

logical

tissues

have

been

obtained

by

the

scanning

electron-acoustlc

thermal

wave

microscopy

by

suitably

adJusting

the

phase.

because

the

acoustic

signals

produced

by

the

thermal

sources

at

dlfferent

depths

are

with

dtfferent

phases.

On

the

other

hand

, m

order

to

theoretically

slmulate

the

laminated

lmagmg

by

the

electron-acoustic

mlcroscopy

,

thermal

conduction

and

thermo-elastic

equations

are

introduced

to

obtam

the

distribut

lO

ns

of

the

temperature

fleld

and

then

the

acoustlC field

in

the

samples.

Based

on

the

solutions

of

the

equatlons

and

related

boundary

condit

lO

ns.

the

depths

of

laminated

image~

can

be

theoretlcally

estlmated

quantltatlvely

as

the

thermal

dtffusivities

of

the

samples

are

determmed.

Meanwhile.

the

thermal

diffusivity

of

the

samples

can

also

be

measured

by

the

electron-acoustlc

detection

technique.

For

the

biologlcal

tlssues

(heart

and

liver

of

rats)

the

thermal

diffUS1VltleS

are

measured

,

which

are

in

the

range

of

O.

45 -

O.

65

mm:

/

s.

and

the

measured

depths

are

about

several

tens

micrometers.

Therefore.

the

depth

profiling

of

the

b

lO

loglcal

tissues

ca

口

be

evaluated

quantitatively

by

the

theoretlcal

htting

to

the

expenmental

laminated

lmage

日.

Key

words:

Thermal

wave

technique

,

laminated

lmaging.

scannmg

electron-acoustic

microscopy

利用扫描电子声显微镜技术实现定量分层成像

朱守经

l

，张淑仪

1.

高椿明

l

，张仲宁

1

，水修基

1

，张秀玲

2

，余万东

2

O.

南京大学声学研究所近代声学重点实验室，南京.

210093;

2.

南京鼓楼医院，南京大学医学院，南京，

210008)

摘

要:

电子声热波显微镜技术成像过程中，由于不同深度的热源具有不同的相位与帽值，因此产生的声信号的

相位与幅值对应于不同的深度和结构.由于热波是高衰减波.所以特别适合于表面层附近结构的检测与分析.实验

上利用压电换能器检测声信号，其输出的电信号通过锁相放大器加以放大、并抑制其余不同频率和相位的信号和

噪声，因此可以增强特定频率和相位的声信号输出.根据检测到的不同相位的声信号，即可分析样品不同深度的结

构，因而扫描电子声热波显微镜技术可以实现分层成像.

Foundation

Item:Nat

lO

nal Natural

SClence

Foundauon

of

Chma

(1

0774074)

Received Date:

2009

一

10-08

相.

Correspondmg

Author.

E-m

a!

l:

zhangsy@;

时

u.

edu.

cn

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