J. L. Arredondo-Figueroa et al.
718
nm) light and photo documented in a Multi Genius SyngeneMR apparatus, us-
ing the Gene Snap (ver 6.02.05) software.
To amplified locus Omy207UoG in F1 we used primers 5’-ACC CTA GTC
ATT CAG TCA GG-3’ and 5’-GAT CAC TGT GAT AGA CAT CG-3 from O'-
Connell (1997), with the following amplification conditions: 94˚C –2 min (one
cycle), and 35 cycles at 94˚C –15 sec, 54˚C –15 sec 72˚C –30 sec.
Capillar electrophoresis was carried out in the automated sequencer system
ABI Prism 3100 Avant at Laboratorio Divisional de Biología Molecular de la Di-
visión de Ciencias Biológicas de la Universidad Autónoma Metropolitana Un-
idad Iztapalapa.
The allelic sizes resolved were determined with the Data Collection V 2.0
software and in relation to the size standard LIZ-500 in GeneMapper V 3.5.
2.2. Genetic Characterization and Diversity of the F1
Genetic variability was assessed by determining the following parameters at the
intra-specific level: 1) number of alleles observed (
NA
) and effective number of
alleles (
EffeA
) were determined in Geno Dive 2.0b23 [21]; 2) polymorphic in-
formation content (
PIC
), allele frequencies and c) allele direct count (AC) ob-
served and expected heterozygosity (
H
O
and
H
E
) were determined in Cervus
3.0.3 [22]. To test Hardy-Weinberg equilibrium (H-W) we used Fisher exact test
as implemented in GENEPOP version 4.0.10 [23] and the
F
IS
estimates for each
allele were computed following [24].
P-values
for overall
F
IS
estimate were calcu-
lated by the Markov chain method using the following parameter: dememoriza-
tion 10,000, 1000 batches and 10,000 iterations per batch.
2.3. Experimental Design for Growth Performance of F1
A total of 942 F1 genetically selected juveniles of rainbow trout were introduced
in a RAS at Planta Experimental de Producción Acuícola (PExPA), located at 2
240 meters above sea level (masl) in Mexico City (CDMX), in six 5 m
3
circular
fish tanks connected to recirculating aquaculture system. In each fish tank 157
juveniles were introduced with an initial average total weight (TW) of 37.8 ±
12.05 g and an average total length (TL) of 14.6 ± 1.53 cm. The experiment has a
duration of 317 days.
2.4. Feeding
The rainbow trout were fed with commercial Steelhead food (45% of protein and
16% of lipids; Silver Cup, Toluca, Mexico) with different pellets sizes according
to the average TW and TL of fishes and were partitioned in two daily rations
(10:00 and 16:00 h). The initial food rate was of 2.5% of the total biomass, de-
creasing according to the TW to 2%, 1.5% and finally 1%.
2.5. Length-Weight Relationship
The length-weight relationship was calculated by the formula TW = aTL
b
, where:
TW = total weight; TL = total length; a = is the intercept and b = the slope; the b