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首页zebrafish phosvitin peptide Pt5的抗微生物与免疫调节作用
zebrafish phosvitin peptide Pt5的抗微生物与免疫调节作用
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AMP(Antimicrobial Peptide,抗菌肽)是一种在生物学中具有重要功能的短链多肽,它们广泛存在于生物体,尤其是许多动物和植物的防御系统中,以对抗微生物感染。本文主要关注的是zebrafish(斑马鱼)中的AMP资源——phosvitin(磷酸肌醇)衍生的抗菌肽Pt5。 Phosvitin是斑马鱼体内的一种天然蛋白质,其C端(氨基末端之后的氨基酸序列)的55个残基被发现具有显著的抗菌活性。在实验室条件下,研究已经证实了Pt5能够有效地抑制多种病原体的生长,展示了其在体外抗菌作用的强大能力。然而,关于这种肽在斑马鱼实际生活中的抗微生物效果以及对宿主免疫系统影响的研究相对较少。 本研究通过实验设计,着重探讨了Pt5在成年斑马鱼体内对抗微生物入侵的能力及其可能的免疫调节作用。实验结果显示,Pt5不仅能有效保护斑马鱼免受病原体的侵害,而且可能通过调控斑马鱼的免疫反应,增强其抵抗疾病的能力。这对于理解斑马鱼免疫防御机制以及开发新型抗菌药物或免疫增强剂具有重要意义。 进一步的研究可能包括探究Pt5的特异性靶标、作用机理以及如何在不损害正常生理功能的前提下增强抗微生物效能。同时,斑马鱼作为模式生物,其AMP研究有助于揭示其他物种中类似的防御策略,对于抗生素耐药性的应对以及开发新的抗菌策略具有潜在价值。 这篇发表于"Peptides"期刊的文章提供了关于斑马鱼phosvitin衍生抗菌肽Pt5的最新研究成果,强调了其在生物防御和免疫调控中的作用,为未来的生物防御研究领域提供了重要的参考依据。
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Peptides
37
(2012)
309–313
Contents
lists
available
at
SciVerse
ScienceDirect
Peptides
j
ourna
l
ho
me
pa
ge:
www.elsevier.com/locate/peptides
Antimicrobial–immunomodulatory
activities
of
zebrafish
phosvitin-derived
peptide
Pt5
Yunchao
Ding,
Xuemei
Liu,
Lingzhen
Bu,
Hongyan
Li,
Shicui
Zhang
∗
Laboratory
for
Evolution
&
Development,
Institute
of
Evolution
&
Marine
Biodiversity
and
Department
of
Marine
Biology,
Ocean
University
of
China,
Qingdao
266003,
China
a
r
t
i
c
l
e
i
n
f
o
Article
history:
Received
18
June
2012
Received
in
revised
form
18
July
2012
Accepted
18
July
2012
Available
online
27
July
2012
Keywords:
Zebrafish
Danio
rerio
Phosvitin
Antimicrobial
peptide
Immunomodulator
a
b
s
t
r
a
c
t
A
phosvitin
(Pv)-derived
peptide,
Pt5,
which
consists
of
the
C-terminal
55
residues
of
Pv
in
zebrafish,
has
been
shown
to
function
as
an
antimicrobial
agent
capable
of
killing
microbes
in
vitro.
However,
its
in
vivo
role
in
zebrafish
remains
unknown.
In
this
study,
we
clearly
demonstrated
that
Pt5
protected
adult
zebrafish
from
pathogenic
Aeromonas
hydrophila
attack,
capable
of
significantly
enhancing
the
survival
rate
of
zebrafish
after
the
pathogenic
challenge.
Pt5
also
caused
a
marked
decrease
in
the
numbers
of
A.
hydrophila
in
the
blood,
spleen,
kidney,
liver
and
muscle,
suggesting
that
Pt5
was
able
to
block
multipli-
cation/dissemination
of
A.
hydrophila
in
zebrafish.
Additionally,
Pt5
markedly
suppressed
the
expression
of
the
proinflammatory
cytokine
genes
IL-1ˇ,
IL-6,
TNF-˛
and
IFN-
in
the
spleen
and
head
kidney
of
A.
hydrophila-infected
zebrafish,
but
it
considerably
enhanced
the
expressions
of
the
antiinflammatory
cytokine
genes
IL-10
and
IL-4
in
the
same
tissues.
Taken
together,
these
data
indicate
that
Pt5
plays
a
dual
role
in
zebrafish
as
an
antimicrobial
and
immunomodulatory
agent,
capable
of
protecting
zebrafish
against
pathogenic
A.
hydrophila
through
its
antimicrobial
activity
as
well
as
preventing
zebrafish
from
the
detrimental
effects
of
an
excessive
inflammatory
response
via
modulating
immune
functions.
©
2012
Elsevier
Inc.
All
rights
reserved.
1.
Introduction
Antimicrobial
peptides
(AMPs)
are
an
evolutionarily
conserved
component
of
the
innate
immunity,
and
are
found
among
virtu-
ally
all
classes
of
organisms
including
plants
and
animals
[7,27].
AMPs
are
generally
12
to
50
amino
acids
in
size.
They
have
been
demonstrated
to
be
potent,
broad
spectrum
antibiotics
capable
of
killing
Gram-negative
and
Gram-positive
bacteria,
mycobacteria,
enveloped
viruses,
fungi
and
even
transformed
or
cancerous
cells
[10].
Several
hundred
AMPs
have
been
identified,
but
only
a
few
of
their
roles
in
host
immunity
have
been
studied
[12,21,23,26].
Apart
from
efficient
antimicrobial
activity,
AMPs
also
regulate
immunity
by
functioning
as
immunomodulators.
They
not
only
boost
the
immune
response
to
prevent
infection
but
also
suppress
other
proinflammatory
responses
to
avoid
uncontrolled
inflamma-
tion,
thereby
resulting
in
homeostasis
of
innate
cellular
defenses
within
an
organism
[2,18].
For
example,
human
neutrophil
peptide
HNP
1–3
binds
C1q
and
activates
the
classic
complement
pathway
[19],
increases
the
production
of
TNF-␣
and
IL-1,
and
decreases
Abbreviations:
AMPs,
antimicrobial
peptides;
Pv,
phosvitin;
LPS,
lipopolysaccha-
ride;
LTA,
lipoteichoic
acid;
PGN,
peptidoglycan;
LD
50
,
50%
lethal
dose;
RPS,
relative
percent
survival.
∗
Corresponding
author
at:
Room
205,
Ke
Xue
Guan,
5
Yushan
Road,
Ocean
Uni-
versity
of
China,
Qingdao
266003,
China.
Tel.:
+86
532
82032787.
E-mail
address:
sczhang@ouc.edu.cn
(S.
Zhang).
the
production
of
IL-10
by
monocytes
[3,8,16].
Furthermore,
some
AMPs
such
as
human
cathelicidin
LL-37
synergize
with
cytokines
and
modify
their
immunomodulatory
activity
[5,6,11].
Zebrafish
(Danio
rerio)
is
a
widely
used
model
organism
to
study
vertebrate
development
and
hematopoiesis
but
interest
in
this
model
organism
has
expanded
into
the
fields
of
human
disease,
cancer
and
immunology
in
recent
years
[9,17,28].
Accord-
ingly,
knowledge
regarding
the
immunology
of
zebrafish
has
been
increasingly
growing
[14,17].
For
example,
several
AMPs
includ-
ing
-defensin
and
hepcidin
have
been
identified
in
zebrafish
[30].
Midkine
and
pleiotrophin
in
zebrafish
have
also
been
shown
to
have
bactericidal
properties
[22].
Recently,
we
have
demonstrated
that
egg
yolk
protein
phosvitin
(Pv)
played
a
critical
role
in
the
immunity
of
zebrafish
embryos
via
acting
as
a
pattern
recognition
receptor
and
an
antimicrobial
effector
[25].
In
addition,
a
Pv-derived
peptide,
Pt5,
which
consists
of
the
C-terminal
55
residues
of
Pv,
also
dis-
played
a
remarkable
antimicrobial
activity
generally
comparable
to
that
of
Pv
as
well
as
an
affinity
to
the
microbial
signature
molecules
lipopolysaccharide
(LPS),
lipoteichoic
acid
(LTA)
and
peptidogly-
can
(PGN),
while
its
mutant,
M13,
generated
by
double
mutation
A201G/I203M,
lost
almost
all
the
antimicrobial
activity
and
the
affinity
to
LPS,
LTA
and
PGN
[25].
Despite
these
progresses,
many
questions
remain
uncertain.
We
do
not
know
if
Pt5
can
protect
adult
zebrafish
from
pathogenic
infection
in
vivo;
and
if
so,
can
it
modulate
the
immune
responses?
The
present
study
was
therefore
undertaken
to
answer
these
questions.
0196-9781/$
–
see
front
matter
©
2012
Elsevier
Inc.
All
rights
reserved.
http://dx.doi.org/10.1016/j.peptides.2012.07.014
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