利用HSP27抗体标记的光声纳米探针成像检测神经损伤

论文

53 浏览量更新于2024-08-27 收藏 609KB PDF 举报

身份认证购VIP最低享 7 折!

30元优惠券

资源详情

资源推荐

Imaging of nerve injury with HSP27 antibody-

functionalized photoacoustic nanoprobes

Hongjiang Chen (陈洪江)

, Zhongjiang Chen (陈重江)

Jankun Xu (许建坤)

, and Jun Hu (胡军)

1,2,

Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College,

Shantou 515041, China

MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics,

South China Normal University, Guangzhou 510631, China

*Corresponding author: hjzkm@vip.163.com

Received May 19, 2015; accepted August 28, 2015; posted online October 16, 2015

Early detection and timely treatment of nerve injury is crucial for the repair of nerve function. One week follow-

ing a crush injury, heat shock protein 27 (HSP27) is over-expressed along the entire length of the sciatic nerve.

Herein, we present an approach to detect injured nerves by photoacoustic microscopy after labeling the injured

nerve with HSP27 antibody-conjugated gold nanoparticles. The studies reveal that nanoprobe administration

enabled the detection of injured nerves by photoacoustic microscopy, especially during the early stages within

3–7 days post injury. In conclusion, photoacoustic microscopy combined with antibody-conjugated nanopar-

ticles holds potential for the early detection of nerve injury.

OCIS codes: 170.0170, 170.3880, 170.5120.

doi: 10.3788/COL201513.111701.

Evidence suggests that injured nerves can achieve better

functional recovery when treated in a timely manner

[1]

Therefore, early diagnosis and appropriate treatment is

very important in cases of nerve injury and would likely

lead to better functional restoration. Electromyography

(EMG) is the only approach that can detect injured motor

or sensory nerves. However, EMG is invasive and only

detects abnormalities at approximately 14–21 days after

the injury occurred

[2,3]

. Since tissue and nerves possess

early the same parameters of density and acoustic imped-

ance, conventional methods, such as ultrasound imaging,

X-rays, computed tomography (CT), and magnetic reso-

nance imaging (MRI), cannot provide high contrast imag-

ing sufficient for identifying injured nerves in situ

[2,4,5]

Therefore, a novel and more effective technology for the

early detection of nerve injury is urgently needed.

Advances in nanotechnology have identified promising

candidates that are more suitable for biological and

biomedical applications over a wide variety of disorders.

Nanoparticles (NPs) with unique properties such as small

particle size, novel physicochemical properties, and easy

surface modification are widely used for biomedical pur-

poses

[6,7]

. Targeting ligands, imaging labels, and therapeu -

tic drugs could all be integrated with contrast reagents as

a promising approach for targeted molecular imaging and

therapy

[8,9]

. Due to the photoacoustic effect, photoacoustic

microscopy is a noninvasive imaging modality with high

resolution and high contrast

[10–13]

. It has been successfully

applied to several biomedical applications for obtaining

structural and functional information, including the

detection of hemoglobin oxygen saturation and carboxy-

hemoglobin saturation, monitoring of vascular damage

during pho todynamic therapy treatment of tumors,

noninvasive monitoring of tr aumatic brain injury, and

post-traumatic rehabilitation

[14–17]

. Previous studies have

shown that heat shock protein 27 (HSP27) in peripheral

nerves can protect injured cells by preventing oxidative

stress

[18]

and resisting heat shock

[19]

. Costigan et al. found

that HSP27 was upregulated by 9-foldin the ipsilateral

dorsal root gang lion (DRG) 48 h after axotomy of the sci-

atic nerve

[20]

. Hirata et al. also found that HSP27 localiza-

tion extended from the proximal to the distal part of the

axon and propagated through the entire nerve within one

week after the surgery

[21]

. Therefore, due to the dramatic

upregulation of HSP27 in injured neurons, we hypoth-

esized that HSP27 could act as an effective marker for

early nerve injury. Four-week-old Sprague–Dawley (SD)

rats (male) weighing 80–100 g were used in the experi-

ments. The rats were placed under general anesthesia with

sodium pentobarbital (200 mg∕kg, i.p.) and then the left

sciatic nerve of the rat was crushed at the middle of the

femur for 30 s with Jeweler’s force ps. Control animals

received the same surgical procedure but without crushing

the nerve. Neurons were cultured according to the method

previously described

[20]

. All procedures followed the rules

of the National Animal Protection of China.

A diagram of the photoacoustic microscopy system is

presented in Fig.

1. Briefly, a tunable pulsed laser source

(Vibrant B 532I, Opotek, USA) with wavelengths of 532

and 680–960 nm, a pulse width of 10 ns, and a pulse rep-

etition rate of 10 Hz, was used to provide optical illumi-

nation. The laser beam was focused by an objective lens

through a small hole in the center of the hollow sensor

and a photodiode was used to monitor and calibrate the

beam intensity and stability. The cylindrical lens and the

position sensor device (PSD) acted as an autofocusing

COL 13(11), 111701(2015) CHINESE OPTICS LETTERS November 10, 2015

下载后可阅读完整内容，剩余4页未读，立即下载

weixin_38607971

粉丝: 3
资源: 972

利用HSP27抗体标记的光声纳米探针成像检测神经损伤

Five-wavelength optical-resolution photoacoustic microscopy of blood and lymphatic vessels

High-resolution photoacoustic microscope for rat brain imaging in vivo

Voxel-based morphometric magnetic resonance imaging (MRI) postprocessing

the early second paper about Optical computational imaging

Describe the background information of Significance of analyzing metal-transfer images for quality control and process optimization in detail

帮我检索癫痫核磁共振近五年发表的文献，并简单说明文献内容

MIMO-SAR-mmWave-Imaging-Toolbox-master教程

用yum怎么下载python-setuptools python-imaging python-ldap MySQL-python python-memcached python-urllib

commons-imaging依赖

计算机视觉审稿快的OA期刊

multi-beam imaging matlab

image[image == -2000] = 0

commons-imaging jar包下载

cannot import name _imaging from PIL

commons-imaging库下载

commons-imaging.jar下载

aspose-imaging.jar

commons-imaging提取exif信息

最新资源