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第 卷第 期 中南民族大学学报自然科学版 Vol No
年 月 Journal of SouthCentral University for Nationalities NatSciEdition Mar
收稿日期 通讯作者 李小军男讲师研究方向糖尿病的胰岛素抵抗Email xiaojunlixiaojun
yahoocomcn
作者简介王继勇男副教授研究方向中药的化学修饰材料腐蚀与防护Email wangjiyongsinacom
基金项 目 湖 北 省 自然 科 学 基 金 资 助 项目 FFC 中 南 民 族 大 学 基 本 科 研 业 务 资 助 项 目 CZQ
XTZ
高 糖 高 脂 饮 食 诱 导 大 鼠 胰 岛 素 抵 抗 模 型 和 评 价
王继勇
高云丽
左洛
何敏
陈聪
李小军
武汉理工大学 化学工程学院武汉 中南民族大学 药学院武汉
摘要为研究高糖高脂结合链脲佐菌素STZ诱导大鼠形成胰岛素抵抗动物模型的可行性将 Wistar 大鼠分为
普通饮食高糖高脂饮食和高糖高脂饮食 STZ 共 组每周测体重和空腹血糖第 周对高糖高脂 STZ 组注射
mg kg 的 STZ 液检测注射 STZ 前注射 STZ h 和 周后的糖耐量情况实验后期检测各组空腹胰岛素水平
胰岛素抵抗指数和肝糖原含量结果表明注射 STZ 前高糖高脂组大鼠体重较正常组明显增加空腹血糖略有上
升糖耐量略有下降注射 STZ h 后高糖高脂 STZ 组 min 与 min 血糖差异显著糖耐量较正常组和高糖
高脂组受损严重 周后仍有差异实验后期高糖高脂组与高糖高脂 STZ 组胰岛素水平胰岛素抵抗和肝糖原均
有降低但与正常组相比无显著差异说明高糖高脂饮食 周腹腔注射 mg kg STZ 后继续高糖高脂 周可形
成胰岛素抵抗动物模型
关键词高糖高脂空腹血糖葡萄糖耐量胰岛素抵抗
中图分类号R
文献标识码A文章编号
HighGlucose and HighFat Diet Induced Insulin Resistance
Rat Model and Its Evaluation
Wang Jiyong
Gao Yunli
Zuo Luo
He Min
Chen Cong
Li Xiaojun
School of Chemical Engineering Wuhan University of Technology Wuhan China
College of Pharmacy SouthCentral University for Nationalities Wuhan China
AbstractTo study the feasibility of the highglucose and highfat STZ on the insulin resistance animal model Wistar
rats were divided into three groups and fed with common highglucose and highfat highglucose and highfat STZ diet
Body weights and fasting bloodglucose were measured weeklyAfter weeks mg kg STZ was injected intraperitoneally
to highglucose and highfat STZ groups and the glucose tolerance before injection in h and w after infection were
observedFasting insulin levels homeostasis model assessment of insulin resistance HOMAIR and hepatic glycogen
contents were also evaluatedThe results indicated that before injecting STZ compared with the control group the weight
of the rats fed with highglucose and highfat increased significantly fasting bloodglucose increased slightly glucose
tolerance decreased slightlyAfter injecting STZ for h there was remarkable difference of bloodglucose in highglucose
and highfat STZ group at min and minCompared with the control group and highglucose and highfat group
glucose tolerance of this group was damaged severely after wMoreover insulin levels HOMAIR and hepatic glycogen
contents all declined slightly though no significant differencesSo insulin resistance could be induced by highglucose and
highfat diet for w then intraperitoneal injection mg kg STZ and highglucose and highfat diet for two more weeks
Keywords highglucose and highfat fasting bloodglucose glucose tolerance insulin resistance
目前糖尿病的发病率呈持续上升状态且多以 II 型糖尿病为主而胰岛素抵抗insulin resistance