添加一列label,以“N”标识正常样本,以“C”标识肿瘤样本,这些信息可以从“GSE83148_series_matrix.txt”基因芯片文件的“!Sample_title”行提取,打印并保存df
时间: 2023-10-05 18:12:45 浏览: 94
好的,您的要求已经记录下来了。我来解答您的问题:
针对您的问题,我可以为您提供以下代码:
```python
import pandas as pd
# 读取GSE83148_series_matrix.txt文件
df = pd.read_csv('GSE83148_series_matrix.txt', sep='\t', skiprows=74)
# 从!Sample_title行获取样本标签信息
labels = df.columns.str.extract(r'!Sample_title\t(.*)', expand=False)
# 添加一列label,以“N”标识正常样本,以“C”标识肿瘤样本
df['label'] = ['N' if 'Normal' in label else 'C' for label in labels]
# 打印并保存df
print(df)
df.to_csv('GSE83148_labeled.csv')
```
这段代码会读取“GSE83148_series_matrix.txt”文件,从“!Sample_title”行获取样本标签信息,并为数据框添加一列名为“label”的列,以“N”标识正常样本,以“C”标识肿瘤样本。最后,将结果打印出来并保存为“GSE83148_labeled.csv”文件。
希望这个回答能够解决您的问题,如果还有其他问题,欢迎随时提出!
相关问题
(1)读取“GSE83148_series_matrix.txt”基因芯片文件和平台“GPL570-55999.txt”(跳过注释行),分别命名为df1和df2 (2)清除df1与df2中的重复数据和缺失数据(df1清除存在NaN的行,df2清除“Gene Symbol”列存在NaN的行) (3)df2新增一列“gene”其值为“Gene Symbol”列的第一个基因名,df1的“ID_REF”列重命名为“ID”列,df2保留“ID”和“gene”列然后与df1合并为df,df中gene名相同的记录表达量取平均值,打印df (4)转置df,并在后面添加一列label,以“N”标识正常样本,以“C”标识肿瘤样本,这些信息可以从“GSE83148_series_matrix.txt”基因芯片文件的“!Sample_title”行提取,打印并保存df (5)将“label”列转换为哑变量(k-1),并作为df的最后一列,删除label列 (6)任选两个基因,分别对其表达量进行离差标准化和标准差标准化(结果不需要保存到df中) (7)尝试任选一个基因,离散化其表达量(不需要保存) (8)统计df的每个基因在肿瘤和正常样本上表达量的平均值,并保存为A (9)提前A中表达量差值的绝对值最大的10个基因名
很抱歉,作为一个语言模型,我没有权限读取或处理本地文件。我能够提供的帮助是,根据您的指示生成代码以供您在本地执行。以下是回答您的问题的Python代码:
(1)读取“GSE83148_series_matrix.txt”基因芯片文件和平台“GPL570-55999.txt”(跳过注释行),分别命名为df1和df2
```python
import pandas as pd
df1 = pd.read_csv("GSE83148_series_matrix.txt", sep="\t", skiprows=31)
df2 = pd.read_csv("GPL570-55999.txt", sep="\t", skiprows=31)
```
(2)清除df1与df2中的重复数据和缺失数据(df1清除存在NaN的行,df2清除“Gene Symbol”列存在NaN的行)
```python
df1.drop_duplicates(inplace=True)
df1.dropna(inplace=True)
df2.drop_duplicates(inplace=True)
df2.dropna(subset=["Gene Symbol"], inplace=True)
```
(3)df2新增一列“gene”其值为“Gene Symbol”列的第一个基因名,df1的“ID_REF”列重命名为“ID”列,df2保留“ID”和“gene”列然后与df1合并为df,df中gene名相同的记录表达量取平均值,打印df
```python
df2["gene"] = df2["Gene Symbol"].apply(lambda x: x.split("|")[0])
df1.rename(columns={"ID_REF": "ID"}, inplace=True)
df = pd.merge(df1, df2[["ID", "gene"]], on="ID")
df = df.groupby(["gene"]).mean().reset_index()
print(df)
```
(4)转置df,并在后面添加一列label,以“N”标识正常样本,以“C”标识肿瘤样本,这些信息可以从“GSE83148_series_matrix.txt”基因芯片文件的“!Sample_title”行提取,打印并保存df
```python
sample_title = pd.read_csv("GSE83148_series_matrix.txt", sep="\t", nrows=28, header=None)
sample_title = sample_title.iloc[27,:].str.split("_")
sample_title = sample_title.apply(lambda x: "N" if x[1]=="N" else "C")
df = df.T
df["label"] = sample_title.values
df = df.T
print(df)
df.to_csv("df.csv", index=False)
```
(5)将“label”列转换为哑变量(k-1),并作为df的最后一列,删除label列
```python
dummy = pd.get_dummies(df["label"], drop_first=True)
df = pd.concat([df, dummy], axis=1)
df.drop(columns=["label"], inplace=True)
```
(6)任选两个基因,分别对其表达量进行离差标准化和标准差标准化(结果不需要保存到df中)
```python
from sklearn.preprocessing import scale
# 离差标准化
gene1 = df.loc["Gene1"]
gene1_normalized = (gene1 - gene1.min()) / (gene1.max() - gene1.min())
# 标准差标准化
gene2 = df.loc["Gene2"]
gene2_normalized = scale(gene2)
```
(7)尝试任选一个基因,离散化其表达量(不需要保存)
```python
# 假设选择"Gene3"
gene3 = df.loc["Gene3"]
gene3_discretized = pd.cut(gene3, bins=3, labels=["Low", "Medium", "High"])
```
(8)统计df的每个基因在肿瘤和正常样本上表达量的平均值,并保存为A
```python
A = df.groupby(lambda x: "N" if x.endswith("N") else "C").mean().T
```
(9)提前A中表达量差值的绝对值最大的10个基因名
```python
diff = abs(A["C"] - A["N"])
top10 = diff.nlargest(10).index.tolist()
```
(1)读取“GSE83148_series_matrix.txt”基因芯片文件和平台“GPL570-55999.txt”(跳过注释行),分别命名为df1和df2 (2)清除df1与df2中的重复数据和缺失数据(df1清除存在NaN的行,df2清除“Gene Symbol”列存在NaN的行) (3)df2新增一列“gene”其值为“Gene Symbol”列的第一个基因名,df1的“ID_REF”列重命名为“ID”列,df2保留“ID”和“gene”列然后与df1合并为df,df中gene名相同的记录表达量取平均值,打印df (4)转置df,并在后面添加一列label,以“N”标识正常样本,以“C”标识肿瘤样本,这些信息可以从“GSE83148_series_matrix.txt”基因芯片文件的“!Sample_title”行提取,打印并保存df (5)将“label”列转换为哑变量(k-1),并作为df的最后一列,删除label列 (6)任选两个基因,分别对其表达量进行离差标准化和标准差标准化(结果不需要保存到df中) (7)尝试任选一个基因,离散化其表达量(不需要保存) (8)统计df的每个基因在肿瘤和正常样本上表达量的平均值,并保存为A (9)提前A中表达量差值的绝对值最大的10个基因名¶
很好,以下是你的答案:
(1)
```python
import pandas as pd
df1 = pd.read_csv('GSE83148_series_matrix.txt', sep='\t', skiprows=79)
df2 = pd.read_csv('GPL570-55999.txt', sep='\t', skiprows=31)
```
(2)
```python
df1.dropna(inplace=True)
df2.dropna(subset=['Gene Symbol'], inplace=True)
df2['gene'] = df2['Gene Symbol'].str.split(' /// ').str[0]
df1.rename(columns={'ID_REF': 'ID'}, inplace=True)
df = pd.merge(df1, df2[['ID', 'gene']], on='ID')
df = df.groupby('gene').mean().reset_index()
```
(3)
```python
df = df.T.reset_index()
df.columns = df.iloc[0]
df = df[1:].reset_index(drop=True)
df['label'] = df['!Sample_title'].str.extract(r'(N|C)')
df = df.iloc[:, :-1]
dummy_label = pd.get_dummies(df['label'], prefix='label', drop_first=True)
df = pd.concat([df.iloc[:, :-1], dummy_label], axis=1)
df.drop('!Sample_title', axis=1, inplace=True)
```
(4)
```python
df = df.T.reset_index()
df.columns = df.iloc[0]
df = df[1:].reset_index(drop=True)
df['label'] = df['!Sample_title'].str.extract(r'(N|C)')
df = df.iloc[:, :-1]
dummy_label = pd.get_dummies(df['label'], prefix='label', drop_first=True)
df = pd.concat([df.iloc[:, :-1], dummy_label], axis=1)
df.drop('!Sample_title', axis=1, inplace=True)
```
(5)
```python
from sklearn.preprocessing import LabelEncoder, OneHotEncoder
label_encoder = LabelEncoder()
df['label'] = label_encoder.fit_transform(df['label'])
one_hot_encoder = OneHotEncoder(categories='auto', sparse=False)
dummy_label = one_hot_encoder.fit_transform(df[['label']])
dummy_label = pd.DataFrame(dummy_label, columns=['label_N', 'label_C'])
df = pd.concat([df.iloc[:, :-1], dummy_label], axis=1)
df.drop('label', axis=1, inplace=True)
```
(6)
```python
from sklearn.preprocessing import StandardScaler
# 离差标准化
scaler1 = StandardScaler()
df['gene1_normalized'] = scaler1.fit_transform(df[['gene1']])
# 标准差标准化
scaler2 = StandardScaler(with_std=False)
df['gene2_normalized'] = scaler2.fit_transform(df[['gene2']])
```
(7)
```python
# 离散化
df['gene3_discretized'] = pd.cut(df['gene3'], bins=3, labels=['low', 'medium', 'high'])
```
(8)
```python
A = df.groupby('gene').mean()[['label_N', 'label_C']]
```
(9)
```python
A['diff'] = abs(A['label_N'] - A['label_C'])
top10_genes = A.nlargest(10, 'diff').index.tolist()
```
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