微生物互作共培养分离技术研究：多样互作影响生长，提高分离效率。

Microorganisms in nature exist in complex communities, with cells interacting through a variety of metabolites, influencing each other's growth. When different strains of bacteria are co-cultured, auxotrophic strains can utilize the metabolites produced by other strains for growth. Co-culturing can also alter the microenvironment, stimulate the expression of silent genes, and change the survival state of the strains. In recent years, co-culturing techniques developed based on simulating interactions between strains have been gradually applied to the isolation of uncultured microorganisms and are believed to effectively improve the efficiency of isolating uncultured microorganisms. This review aims to provide a comprehensive analysis of the potential diversity of co-cultured groups and the advanced nature and current applications of co-culture isolation techniques based on published literature, in order to provide reference for the development of microbial isolation techniques and the exploration of microbial resources.