写一个fasta id替换代码,其中新id在txt文件 新id内容包含旧id
时间: 2024-03-27 09:40:54 浏览: 65
好的,你可以参考下面的代码实现fasta id替换,其中新id文件中包含旧id和对应的新id。假设fasta文件路径为"E:\泛基因组分析\ORF\ORF_xml\PRRSV.fasta",新id文件路径为"E:\泛基因组分析\ORF\ORF_xml\PRRSV_newid.txt":
```
import os
fasta_file = "E:\泛基因组分析\ORF\ORF_xml\PRRSV.fasta"
new_id_file = "E:\泛基因组分析\ORF\ORF_xml\PRRSV_newid.txt"
if not os.path.exists(fasta_file):
print("Error: Fasta file does not exist!")
exit()
if not os.path.exists(new_id_file):
print("Error: New id file does not exist!")
exit()
new_ids = {}
try:
with open(new_id_file, "r", encoding="utf-8") as f:
for line in f:
old_id, new_id = line.strip().split()
new_ids[old_id] = new_id
except:
print("Error: Failed to read new id file!")
exit()
try:
with open(fasta_file, "r", encoding="utf-8") as f:
lines = f.readlines()
except:
print("Error: Failed to read fasta file!")
exit()
new_lines = []
for line in lines:
if line.startswith(">"):
# 获取当前id
old_id = line.strip().lstrip(">")
# 替换为新id
if old_id in new_ids:
new_id = new_ids[old_id]
new_lines.append(">{}\n".format(new_id))
else:
print("Error: Id not found in new id file! ({})".format(old_id))
exit()
else:
new_lines.append(line)
# 将替换后的fasta文件写入新文件
new_fasta_file = "E:\泛基因组分析\ORF\ORF_xml\PRRSV_new.fasta"
with open(new_fasta_file, "w") as f:
f.writelines(new_lines)
print("Done!")
```
新id文件中每行包含一个旧id和一个新id,通过读取新id文件构建一个字典new_ids,将旧id作为字典的键,将新id作为字典的值。在处理fasta文件时,如果当前行是id行,则获取当前id,从字典中查找对应的新id并替换。如果当前id在字典中不存在,则输出错误信息并退出程序。最后将替换后的fasta文件写入新文件。
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