帮我为下面的代码加上注释：class SimpleDeepForest: def __init__(self, n_layers): self.n_layers = n_layers self.forest_layers = [] def fit(self, X, y): X_train = X for _ in range(self.n_layers): clf = RandomForestClassifier() clf.fit(X_train, y) self.forest_layers.append(clf) X_train = np.concatenate((X_train, clf.predict_proba(X_train)), axis=1) return self def predict(self, X): X_test = X for i in range(self.n_layers): X_test = np.concatenate((X_test, self.forest_layers[i].predict_proba(X_test)), axis=1) return self.forest_layers[-1].predict(X_test[:, :-2]) # 1. 提取序列特征（如：GC-content、序列长度等） def extract_features(fasta_file): features = [] for record in SeqIO.parse(fasta_file, "fasta"): seq = record.seq gc_content = (seq.count("G") + seq.count("C")) / len(seq) seq_len = len(seq) features.append([gc_content, seq_len]) return np.array(features) # 2. 读取相互作用数据并创建数据集 def create_dataset(rna_features, protein_features, label_file): labels = pd.read_csv(label_file, index_col=0) X = [] y = [] for i in range(labels.shape[0]): for j in range(labels.shape[1]): X.append(np.concatenate([rna_features[i], protein_features[j]])) y.append(labels.iloc[i, j]) return np.array(X), np.array(y) # 3. 调用SimpleDeepForest分类器 def optimize_deepforest(X, y): X_train, X_test, y_train, y_test = train_test_split(X, y, test_size=0.2) model = SimpleDeepForest(n_layers=3) model.fit(X_train, y_train) y_pred = model.predict(X_test) print(classification_report(y_test, y_pred)) # 4. 主函数 def main(): rna_fasta = "RNA.fasta" protein_fasta = "pro.fasta" label_file = "label.csv" rna_features = extract_features(rna_fasta) protein_features = extract_features(protein_fasta) X, y = create_dataset(rna_features, protein_features, label_file) optimize_deepforest(X, y) if __name__ == "__main__": main()

# Define a class named 'SimpleDeepForest'
class SimpleDeepForest:

# Initialize the class with 'n_layers' parameter
def __init__(self, n_layers):
self.n_layers = n_layers
self.forest_layers = []

# Define a method named 'fit' to fit the dataset into the classifier
def fit(self, X, y):
X_train = X

# Use the forest classifier to fit the dataset for 'n_layers' times
for _ in range(self.n_layers):
clf = RandomForestClassifier()
clf.fit(X_train, y)

# Append the classifier to the list of forest layers
self.forest_layers.append(clf)

# Concatenate the training data with the predicted probability of the last layer
X_train = np.concatenate((X_train, clf.predict_proba(X_train)), axis=1)

# Return the classifier
return self

# Define a method named 'predict' to make predictions on the test set
def predict(self, X):
X_test = X

# Concatenate the test data with the predicted probability of each layer
for i in range(self.n_layers):
X_test = np.concatenate((X_test, self.forest_layers[i].predict_proba(X_test)), axis=1)

# Return the predictions of the last layer
return self.forest_layers[-1].predict(X_test[:, :-2])

# Define a function named 'extract_features' to extract sequence features
def extract_features(fasta_file):
features = []

# Parse the fasta file to extract sequence features
for record in SeqIO.parse(fasta_file, "fasta"):
seq = record.seq
gc_content = (seq.count("G") + seq.count("C")) / len(seq)
seq_len = len(seq)
features.append([gc_content, seq_len])

# Return the array of features
return np.array(features)

# Define a function named 'create_dataset' to create the dataset
def create_dataset(rna_features, protein_features, label_file):
labels = pd.read_csv(label_file, index_col=0)
X = []
y = []

# Create the dataset by concatenating the RNA and protein features
for i in range(labels.shape[0]):
for j in range(labels.shape[1]):
X.append(np.concatenate([rna_features[i], protein_features[j]]))
y.append(labels.iloc[i, j])

# Return the array of features and the array of labels
return np.array(X), np.array(y)

# Define a function named 'optimize_deepforest' to optimize the deep forest classifier
def optimize_deepforest(X, y):
# Split the dataset into training set and testing set
X_train, X_test, y_train, y_test = train_test_split(X, y, test_size=0.2)

# Create an instance of the SimpleDeepForest classifier with 3 layers
model = SimpleDeepForest(n_layers=3)

# Fit the training set into the classifier
model.fit(X_train, y_train)

# Make predictions on the testing set
y_pred = model.predict(X_test)

# Print the classification report
print(classification_report(y_test, y_pred))

# Define the main function to run the program
def main():
rna_fasta = "RNA.fasta"
protein_fasta = "pro.fasta"
label_file = "label.csv"

# Extract the RNA and protein features
rna_features = extract_features(rna_fasta)
protein_features = extract_features(protein_fasta)

# Create the dataset
X, y = create_dataset(rna_features, protein_features, label_file)

# Optimize the DeepForest classifier
optimize_deepforest(X, y)

# Check if the program is being run as the main program
if __name__ == "__main__":
main()